Juvenile | Adult | |
---|---|---|
Firing pattern | ||
Delay first AP (ms) | 462 ± 57 | 395 ± 79 |
ISI average (ms) | 25.9 ± 1.2 | 24.2 ± 1.4 |
Frequency adaptation | 1.03 ± 0.12 | 1.30 ± 0.09* |
Max ISI (ms) | 109 ± 23 | 120 ± 40 |
Min ISI (ms) | 20.3 ± 1.3 | 18.4 ± 1.3 |
Max/Min ISI | 6.4 ± 1.7 | 7.6 ± 2.6 |
CV frequency | 0.20 ± 0.02 | 0.20 ± 0.03 |
F/I slope (Hz/pA) | 2.2 ± 0.3 | 1.2 ± 0.1*** |
Passive | ||
Vm (mV) | -86 ± 1.4 | -87 ± 1.2 |
Rm (MΩ) | 106 ± 10 | 159 ± 12*** |
Rheobase (pA) | 384 ± 46 | 228 ± 16** |
AP shape | ||
AP threshold (mV) | -46.3 ± 1.5 | -49.6 ± 1.0* |
AP 10-90% rise (ms) | 0.27 ± 0.01 | 0.25 ± 0.01 |
AP half-width (ms) | 0.54 ± 0.04 | 0.44 ± 0.03* |
AHP peak (mV) | -24.7 ± 0.6 | -23.8 ± 1.0 |
AHP latency (ms) | 2.2 ± 0.4 | 1.4 ± 0.2* |
AHP area (V/s) | -298 ± 42 | -213 ± 15* |
Number of interneurons | 14 | 20 |
-
↵Juvenile (P15-P17) or adult (P56-P65) PV interneurons are characterized by firing pattern, passive membrane properties, and AP shape (see Materials and Methods). Values shown as mean ± SEM. A t test was used to determine whether the value for juvenile was significantly different from that for adult (*p < 0.05, **p < 0.01, and ***p < 0.001). Note that the number of recorded interneurons is less than sampled for mRNA (19, juvenile; 21, adult) because not all interneurons sampled provided stable AP recordings.